Its genomic dna was partially digested by sauial . dna fragments from 4 to 16 kb were collected after electrophoresis and ligated with bamhi - digested puc18 to construct genomic library . the total number of recombinant plasmids is about 9000 进一步在opua基因的上下游序列分别设计引物，从h . trueperi基因组dna中扩增出所预期大小的片段，测序验证已获得opua基因的全序列。
This has resulted in the availability of genetically modified l . lactis with prospects for application in food industry . in this studay , genomic dna extracted from lactococcus lactis nizo r5 was used directly as the template for pcr in this paper 乳酸乳球菌是一类长期应用于食品发酵业的有益微生物，以乳酸菌为受体的表达系统具有安全、表达产物便于分离，使用方便等优点。
300 clear bands including 289 polymorphic bands and 50 specific bands were amplified from the genomic dna of gastrodia elata bl . by these selected primers . the rate of polymorphic bands and the rate of specific bands were 98 . 0 % and 16 . 6 % respectively 6个引物组合共扩增出300条清晰可辨的条带，其中289条多态性条带， 50条特异性条带；多态性条带率和特异性条带率分别达98
In the same method , another pair of primers weredesigned in order to amplify subtilisin carlsbsrg from genomic dna of those bacillus strains by pcr . the cloning and sequencing of a fragment from bacillus licheniformis dy . are under investigation 又用扩增地衣芽孢杆菌碱性蛋白酶基因的引物在b . licheniformisdy的总dna中扩增到一条与预期大小相一致的片段，进一步实验仍在进行中。
Comparison of multimegabase genomic dna sequences is a popular technique for finding and annotating conserved genome features . performing such comparisons entails finding many short local alignments between sequences up to tens of megabases in length 比较数兆碱基的dna序列是发现和标记保守的基因组特征普遍使用的技术，进行这样的比较可以发现长达几十兆的序列之间很多短的局部对齐。
Designing a specific primer pair based on the sequence of the gene ubia in e . coli mc4100 , a dna fragment was amplified from the genomic dna of e . coli mc4100 by pcr and then was cloned into pucm - t vector . the cloned fragment was sequenced 根据e . colimc4100的ubia基因全碱基顺序，合理设计引物，以e . colimc4100基因组dna为模板进行pcr扩增，将pcr产物克隆于pucm - tvector后对其进行测序鉴定。
A pair of primers , based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus , were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction Sds 、 edta和pmsf对酶活力有不同程度的抑制作用。以4株芽孢杆菌的总dna为模版，利用扩增短小芽孢杆菌碱性蛋白酶基因的引物对它们进行pcr扩增。
The results showed that mod dna and cdna were 1378 bp and 853 bp in size respectively . for the first time , three introns were found in mod genomic dna by comparing dna to cdna , whose size were 315 bp , 123 bp and 87 bp respectively 测序西南农业人学硕士学位论文结果表明，人介gddxa大小为1378bp ，相对于edna853bp来说多了525bp ，在dna中首次发现了3个内含子，大小分别为315bp ， 123bp和87bp 。
Cloning and sequence analysis of thl2 gene using the genomic dna and stigma total rna isolated from b . olereacea l . and b . napus l . as templates in pcr and rt - pcr reaction , the thl2 dna and cdna were amplified , recovered , cloned into vectors and sequenced Thl2基因的克隆与序列分析提取甘蓝和油菜的基因组dna和柱头总rna ，采用pcr和rt - pcr技术扩增thl2dna和cdna ，对目的带进行回收，克隆并测序。
Over the past few years , although many factors have been put forward to explain this phenomenon , and even though the complete genomic dna sequence of d . radiodurans is now known , the molecular mechanism underlying this phenotype is still poorly understood 自该细菌发现以来，科学家们提出各种假设试图来解释这种现象，即使全基因组测序工作也已经完成，但耐辐射球菌真正的抗辐射机制仍然是个谜。
